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> Dear Confocalists,
>
> for teaching purposes, I would like to have a graph with the following:
>
> 1) Intensity profile through the center of the Airy pattern (x,y) of a
> fluorescent point for a wavelength of 500 nm (as, say, mean between 488
> exc. and 512 em) in widefield, with the distance from the maximum on the
> x-axis in nano- or micrometers and the normalized intensity on the
> y-axis, say for a NA 1.4 Objective.
>
> 2) Same for confocal, closed pinhole.
>
> Bonus:
> 3) Same for confocal/two photon with 900 nm excitation
> 4) All of the above along the optical axis instead of the x,y plane.
>
>
> One purpose of this graph (more precisely: comparison of 1 and 2) would
> be to visualize with typical parameters why in the confocal/two-photon
> case with the steeper fall in intensity two Airy patterns can be closer
> together and still have a 26% dip in intensity between them (called
> extended Rayleigh-criterion, I believe?) although the ring with the
> first minimum has the same diameter.
>
> I am sure I could find that or a similar version in some book or
> article, however I would like to include this in material published on
> the web. And to avoid copyright issues the graphs have to be in the
> public domain or with a Creative Commons Attribution License (CC-by).
> Which probably means that they have to be made new. I would be perfectly
> happy to make such graphs myself if I had the respective data (i.e.
> columns with numbers for the x- and Y-values) to generate them in Excel,
> Calc or Graphpad. However, as a mere biologist I don't know how to
> generate such columns.
>
> Therefore I would like to ask if someone would be willing to help out
> and generate such columns with numbers. I assume that it is not that
> much work for somebody with good insight in Airy pattern formulas and
> the right program at hands - and hope I am not wrong with that
> assumption. In particular data for graphs 1 and 2 above would be very
> helpful.
>
> I am thinking that covering the distance from -5 to +5 µm in 10 nm steps
> would probably be more than sufficient.
>
> Cheers
>
> Steffen
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
>
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