Re: Numbers for Airy pattern graphs?

Posted by David Baddeley on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Leica-TCS-SP2-LCS-and-Win7-tp7582853p7582935.html

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Dear Steffen,
just to add to the suggestions already made, confocal and two-photon PSFs are really easy to derive from an emission or excitation case, if you have a psf generation program for widefield.
To a 1st appoximation (i.e. infinitely small pinhole):
confocal PSF = widefield PSF at exctitation wavelength multiplied by widefield PSF at detection wavelength
Or for a large pinhole in 2-photon
2-photon PSF = widefield PSF at excitation wavelength squared.
These multiplications should be easy to do in ImageJ (convert the data type to floating point first)
If you want to model a finite pinhole size you can convolve the detection PSF with a flat disc with the pinhole diameter before doing the multiplication (doable in ImageJ, but somewhat harder, as the disc needs to be centred to avoid shifting the excitation and detection PSFS wrt each other).
cheers,David