Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/inter-manufacturer-objective-compatibility-tp7582994p7583001.html
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Maurice,
we have a LaVision Biotec multi-photon which is built around an Olympus
stand. A few years ago bought additional objectives, to supplement the
(very good) 20x 0.95 Objective from Olympus for specific tasks. After
testing several ones we ended up buying one from Leica (40x, 1.1, better
resolution, but flat top) and the 16x Nikon (steeper angle, could access
places the others couldn't). Putting all theory aside, they both work
nicely for their specific purpose.
Steffen
Am 26.11.2014 19:23, schrieb Needham, Maurice:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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>
> Hi Everyone,
>
> We have an Olympus two-photon system that we would like to use a Nikon 16X lens (CFI LWD 16XW, NA 0.8, WD 3mm) with. We realize that the beam expander on the Olympus will not compensate for this lens but wondered if anyone has experience with “fooling” the software to allow compensation when using non-Olympus lenses. Also, we would appreciate comments on the utility of this lens for two-photon imaging as we also have a custom system that won’t have the beam expander problem.
>
> In addition, does anyone know of a supplier or accurate method of fabricating adaptors to allow use of objective lens between different manufacturer's scopes. Different objective screw thread is the main issue.
>
> Thanks for your help,
>
> Maurice
>
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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