Commercial solutions for calibration of FRAP units

Posted by Anton Kamnev on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034.html

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Dear all,

In our LM core we face a problem of frequent calibration of our "FRAP"
devices. Although the calibration itself is quite fast, many users just
don't have good samples which could be used for this purpose.

Therefore, I was wondering if there are any commercial products for
calibration of bleaching devices? What I'm looking for is a slide with a
thin uniform layer of a dye which could be bleached. The dye should be
immobilized so a bleaching pattern would be observable for some period of
time after the bleaching.

I would really appreciate if you could share some experience about samples
you use for calibration of bleaching patterns.

Although I'm primarily interested in commercial solutions, I would very much
appreciate some protocols for home-made calibration slides as well.

Thanks in advance!
Anton.

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Anton Kamnev, PhD
Imaging Manager
Mechanochemical Cell Biology Building
Division of Biomedical Cell Biology
Warwick Medical School
The University of Warwick
Coventry, CV4 7AL UK

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