Re: Commercial solutions for calibration of FRAP units - COMMERCIAL RESPONSE

Posted by WAINWRIGHT James on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034p7583037.html

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COMMERCIAL RESPONSE

Hi everyone,

Andor manufacture and supply "FRAPPA Calibration Slides" with our FRAPPA units.

They are thin gels of Ethidium Bromide. I'd imagine they're easy to make?

Our part code is XS-AND-001 and can be requested from your regional Microscopy Sales Engineers:

http://www.andor.com/map.aspx

I'm not sure of the cost for these, but I'd imagine it's pretty low. (I'm unable to provide costs because I am located in Technical Support and we have limited access to the price lists).

Best regards,

James

James Wainwright
Product Support Engineer (Microscopy Systems)
Europe, Middle East & Africa

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Web:  andor.com/support



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anton Kamnev
Sent: 01 December 2014 13:41
To: [hidden email]
Subject: Commercial solutions for calibration of FRAP units

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Dear all,

In our LM core we face a problem of frequent calibration of our "FRAP"
devices. Although the calibration itself is quite fast, many users just don't have good samples which could be used for this purpose.

Therefore, I was wondering if there are any commercial products for calibration of bleaching devices? What I'm looking for is a slide with a thin uniform layer of a dye which could be bleached. The dye should be immobilized so a bleaching pattern would be observable for some period of time after the bleaching.

I would really appreciate if you could share some experience about samples you use for calibration of bleaching patterns.

Although I'm primarily interested in commercial solutions, I would very much appreciate some protocols for home-made calibration slides as well.

Thanks in advance!
Anton.

---------------------------
Anton Kamnev, PhD
Imaging Manager
Mechanochemical Cell Biology Building
Division of Biomedical Cell Biology
Warwick Medical School
The University of Warwick
Coventry, CV4 7AL UK

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Divisional Secretary: Karl Waldron
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