Re: Commercial solutions for calibration of FRAP units

Posted by phil laissue-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034p7583038.html

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Donnert, Eggeling & Hell 2007 made a monolayer of Atto532 NHS ester on a
silanized coverslip, or alternately used purified GFP and Venus in a
~500-nm adsorbed layer (poly-L-lysine) and Mowiol-mounted.
http://www.ncbi.nlm.nih.gov/pubmed/17179937

_________________________________________
Philippe Laissue, PhD, Director of Bioimaging Unit
School of Biological Sciences, Room 4.17
University of Essex, Colchester CO4 3SQ, UK
(0044) 01206 872246 / (0044) 07842 676 456
[hidden email]
privatewww.essex.ac.uk/~plaissue

On 1 December 2014 at 13:40, Anton Kamnev <[hidden email]> wrote:

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>
> Dear all,
>
> In our LM core we face a problem of frequent calibration of our "FRAP"
> devices. Although the calibration itself is quite fast, many users just
> don't have good samples which could be used for this purpose.
>
> Therefore, I was wondering if there are any commercial products for
> calibration of bleaching devices? What I'm looking for is a slide with a
> thin uniform layer of a dye which could be bleached. The dye should be
> immobilized so a bleaching pattern would be observable for some period of
> time after the bleaching.
>
> I would really appreciate if you could share some experience about samples
> you use for calibration of bleaching patterns.
>
> Although I'm primarily interested in commercial solutions, I would very
> much
> appreciate some protocols for home-made calibration slides as well.
>
> Thanks in advance!
> Anton.
>
> ---------------------------
> Anton Kamnev, PhD
> Imaging Manager
> Mechanochemical Cell Biology Building
> Division of Biomedical Cell Biology
> Warwick Medical School
> The University of Warwick
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