Re: Commercial solutions for calibration of FRAP units

Posted by mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034p7583041.html

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p.s.  Since these web pages over ten years ago, we added measuring the intensity at the sample with a power meter before doing anything like this.

=========================================================================
 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
                          Cell:  914-309-3270     Temporary location:  SK2-7
          http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cammer, Michael
Sent: Monday, December 01, 2014 10:33 AM
To: [hidden email]
Subject: Re: Commercial solutions for calibration of FRAP units

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We've used these slides for this.
e.g. http://cammer.net/historical/aif/instructions/zeiss/liveduo/Zaxis_bleachingtest/index.htm
http://cammer.net/historical/aif/instructions/fluor-ref-slides/01.htm


=========================================================================
 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
                          Cell:  914-309-3270     Temporary location:  SK2-7
          http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: Monday, December 01, 2014 9:07 AM
To: [hidden email]
Subject: Re: Commercial solutions for calibration of FRAP units

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Dear Anton,

Just a quick idea: the plastic fluorescent slides distributed by Chroma (but also others) can be also bleached (if you have a somewhat stronger laser). Could this work for you?

Greetings    Gabor

On 12/1/14 2:40 PM, "Anton Kamnev" <[hidden email]> wrote:

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>
>Dear all,
>
>In our LM core we face a problem of frequent calibration of our "FRAP"
>devices. Although the calibration itself is quite fast, many users just
>don't have good samples which could be used for this purpose.
>
>Therefore, I was wondering if there are any commercial products for
>calibration of bleaching devices? What I'm looking for is a slide with
>a thin uniform layer of a dye which could be bleached. The dye should
>be immobilized so a bleaching pattern would be observable for some
>period of time after the bleaching.
>
>I would really appreciate if you could share some experience about
>samples you use for calibration of bleaching patterns.
>
>Although I'm primarily interested in commercial solutions, I would very
>much appreciate some protocols for home-made calibration slides as
>well.
>
>Thanks in advance!
>Anton.
>
>---------------------------
>Anton Kamnev, PhD
>Imaging Manager
>Mechanochemical Cell Biology Building
>Division of Biomedical Cell Biology
>Warwick Medical School
>The University of Warwick
>Coventry, CV4 7AL UK
>
>tel: [phone]
>cell: [phone]
>email: [hidden email]
>
>Divisional Secretary: Karl Waldron
>Tel: [phone]
>[hidden email]