Re: Commercial solutions for calibration of FRAP units

Posted by Lange, Jeffrey on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034p7583042.html

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Hello all-

I commonly mark the inside of a Mat Tek dish with a red sharpie marker. This gives a very flat uniform field that is easily imaged and bleached with a 561nm laser. There is no recovery of the marker over the course of months.

Jeff

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anton Kamnev
Sent: Monday, December 01, 2014 7:41 AM
To: [hidden email]
Subject: Commercial solutions for calibration of FRAP units

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Dear all,

In our LM core we face a problem of frequent calibration of our "FRAP"
devices. Although the calibration itself is quite fast, many users just don't have good samples which could be used for this purpose.

Therefore, I was wondering if there are any commercial products for calibration of bleaching devices? What I'm looking for is a slide with a thin uniform layer of a dye which could be bleached. The dye should be immobilized so a bleaching pattern would be observable for some period of time after the bleaching.

I would really appreciate if you could share some experience about samples you use for calibration of bleaching patterns.

Although I'm primarily interested in commercial solutions, I would very much appreciate some protocols for home-made calibration slides as well.

Thanks in advance!
Anton.

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Anton Kamnev, PhD
Imaging Manager
Mechanochemical Cell Biology Building
Division of Biomedical Cell Biology
Warwick Medical School
The University of Warwick
Coventry, CV4 7AL UK

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