Posted by
Stanislav Vitha-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/inter-manufacturer-objective-compatibility-tp7582994p7583044.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
On Wed, 26 Nov 2014 19:21:50 +0000, Andreas Bruckbauer
<
[hidden email]> wrote:
>The beam expander on the newer Olympus multiphotons is just that, it is
motorized and makes sure the back aperture is filled when using different
objectives. You can just define a new objective with the right magnification
and NA to 'fool' it. This way it is also possible to underfill existing objectives.
>Best wishes
>Andreas
At least on our single-photon Olympus FV1000 (software v. 3.1), there is a
plain text file somewhere in the Fluoview installation folder that contains
the list of objectives. You can edit the file to add definition of another
objective. If I remember correctly, it contains information like
magnification, NA, immersion medium, working distance, back aperture
diameter, ..
I did that when testing a Zeiss 25x/0.8 multiimmersion objective on the
Olympus (worked, but as expected had a pretty significant chromatic
aberration). I do not remember the name of the definition file.
Stan Vitha
Texas A&M University
Microscopy and Imaging Center