Re: Commercial solutions for calibration of FRAP units

Posted by Barbara Foster on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034p7583047.html

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There are also Fluor-Ref slides from us, at www.MicroscopyEducation.com

These are the slides originally invented by P C Cheng.

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At 07:48 AM 12/1/2014, Jorge wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>We successfully used this product from Chroma (no commercial interest)
>
>Autofluorescent Plastic Slides
>
>(link https://www.chroma.com/products/filter-accessories/diagnostic-slides)
>
>Hope that helps,
>
>Jorge
>
>2014-12-01 10:40 GMT-03:00 Anton Kamnev <[hidden email]>:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your posting.
> > *****
> >
> > Dear all,
> >
> > In our LM core we face a problem of frequent calibration of our "FRAP"
> > devices. Although the calibration itself is quite fast, many users just
> > don't have good samples which could be used for this purpose.
> >
> > Therefore, I was wondering if there are any commercial products for
> > calibration of bleaching devices? What I'm looking for is a slide with a
> > thin uniform layer of a dye which could be bleached. The dye should be
> > immobilized so a bleaching pattern would be observable for some period of
> > time after the bleaching.
> >
> > I would really appreciate if you could share some experience about samples
> > you use for calibration of bleaching patterns.
> >
> > Although I'm primarily interested in commercial solutions, I would very
> > much
> > appreciate some protocols for home-made calibration slides as well.
> >
> > Thanks in advance!
> > Anton.
> >
> > ---------------------------
> > Anton Kamnev, PhD
> > Imaging Manager
> > Mechanochemical Cell Biology Building
> > Division of Biomedical Cell Biology
> > Warwick Medical School
> > The University of Warwick
> > Coventry, CV4 7AL UK
> >
> > tel: [phone]
> > cell: [phone]
> > email: [hidden email]
> >
> > Divisional Secretary: Karl Waldron
> > Tel: [phone]
> > [hidden email]
> >
>
>
>
>--
>Jorge Toledo H
>Ph.D
>Biomedical Neuroscience Institute (BNI)
>Laboratory of cellular and molecular neurobiology(Couve-Lab)
>Laboratory of Scientific Image Analysis (SCIAN-Lab)
>Faculty of Medicine
>University of Chile
>www.couvelab.org | www.scian.cl