http://confocal-microscopy-list.275.s1.nabble.com/Commercial-solutions-for-calibration-of-FRAP-units-tp7583034p7583050.html
We use blue ink (permanent marker) on a coverslip with a small drop of water on the slide. You can focus de ink and bleach it.
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> Dear all,
>
> In our LM core we face a problem of frequent calibration of our "FRAP"
> devices. Although the calibration itself is quite fast, many users just
> don't have good samples which could be used for this purpose.
>
> Therefore, I was wondering if there are any commercial products for
> calibration of bleaching devices? What I'm looking for is a slide with a
> thin uniform layer of a dye which could be bleached. The dye should be
> immobilized so a bleaching pattern would be observable for some period of
> time after the bleaching.
>
> I would really appreciate if you could share some experience about samples
> you use for calibration of bleaching patterns.
>
> Although I'm primarily interested in commercial solutions, I would very much
> appreciate some protocols for home-made calibration slides as well.
>
> Thanks in advance!
> Anton.
>
> ---------------------------
> Anton Kamnev, PhD
> Imaging Manager
> Mechanochemical Cell Biology Building
> Division of Biomedical Cell Biology
> Warwick Medical School
> The University of Warwick
> Coventry, CV4 7AL UK
>
> tel: +44 (0) 24-7615-1934
> cell: +44 (0) 782-408-6941
> email:
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> Divisional Secretary: Karl Waldron
> Tel: +44(0) 24-7615-1154
>
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