shrinkage of cells druing fixation

Posted by Jeremy Adler-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shrinkage-of-cells-druing-fixation-tp7583101.html

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Cells on coverslips tend to flatten during fixation.
In LM the Z resolution is always poorer than in XY and shrinkage in Z exacerbates this mismatch.
Does anyone have any suggestions for preventing or reducing shrinkage.