Re: shrinkage of cells druing fixation

Posted by JOEL B. SHEFFIELD on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shrinkage-of-cells-druing-fixation-tp7583101p7583105.html

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Many years ago, I examined the effect of changing the osmolarity of my
fixative on the size distribution of dissociated retinal cells.  Although
this work was done with glutaraldehyde fixatives for electron microscopy,
it still seems to be valid.  We found that for those cells, the optimal
fixation was in 0.08M phosphate buffer, with 2.5% glutaraldehyde.  The
paper was published in Developmental Biology 23:36-61 (1970)

Joel



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  *http://tinyurl.com/khbouft <http://tinyurl.com/khbouft>*

On Tue, Dec 9, 2014 at 5:52 AM, Jeremy Adler <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Cells on coverslips tend to flatten during fixation.
> In LM the Z resolution is always poorer than in XY and shrinkage in Z
> exacerbates this mismatch.
> Does anyone have any suggestions for preventing or reducing shrinkage.
>