Re: shrinkage of cells druing fixation

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shrinkage-of-cells-druing-fixation-tp7583101p7583108.html

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Jeremy,

independent of the fixation itself, you should also have an eye on your
embedding medium. Hardening medium such as Mowiol or ProLong tends to
flatten the cells quite a bit, compared to non-hardening such as
Vectashield.

Steffen

Am 09.12.2014 11:52, schrieb Jeremy Adler:

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> Cells on coverslips tend to flatten during fixation.
> In LM the Z resolution is always poorer than in XY and shrinkage in Z exacerbates this mismatch.
> Does anyone have any suggestions for preventing or reducing shrinkage.
>

--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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