High speed spinning disc confocal with EMCCD camera

Posted by sivaram mylavarapu on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142.html

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Dear colleagues,

We are planning on acquiring a high-speed, high-sensitivity confocal
fluorescence imaging station for meeting two simultaneous needs: 1) high
speed for live cell imaging and 2) very high sensitivity to be able to
capture very low light images. We have been seriously considering a
spinning disc confocal with an EMCCD camera attachment to meet both these
needs. We would of course include halogen illumination for routine
widefield fluorescence imaging.

I wanted to request your opinions on the following:

1) Which is the best microscope in the market for these needs? I have used
a Zeiss spinning disc in the past, and it appears Leica has an instrument
too - both use Yokogawa spinning discs. Nikon sells their system with an
Andor spinning disc.

2) Which is the best EMCCD camera for such a system?

3) Can I combine the above features to be supplied with a dual inverted and
upright microscope so that both high resolution live cell (inverted) and C
elegans/ Zebrafish/ Drosophila embryo live imaging (upright) can be
performed as per need?

I would appreciate your valuable inputs!

Warm regards,

Sivaram.
--
Sivaram Mylavarapu.
[hidden email]