http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583143.html
In regards to point 3) that you raised, for such a setup, I think you'll need two spinning disk scan heads... Although with a fancy set of switchable image relays, you might be able to share one scan head for the upright and inverted sections of the microscope. Never seen anything like that attempted though.
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> Dear colleagues,
>
> We are planning on acquiring a high-speed, high-sensitivity confocal
> fluorescence imaging station for meeting two simultaneous needs: 1) high
> speed for live cell imaging and 2) very high sensitivity to be able to
> capture very low light images. We have been seriously considering a
> spinning disc confocal with an EMCCD camera attachment to meet both these
> needs. We would of course include halogen illumination for routine
> widefield fluorescence imaging.
>
> I wanted to request your opinions on the following:
>
> 1) Which is the best microscope in the market for these needs? I have used
> a Zeiss spinning disc in the past, and it appears Leica has an instrument
> too - both use Yokogawa spinning discs. Nikon sells their system with an
> Andor spinning disc.
>
> 2) Which is the best EMCCD camera for such a system?
>
> 3) Can I combine the above features to be supplied with a dual inverted and
> upright microscope so that both high resolution live cell (inverted) and C
> elegans/ Zebrafish/ Drosophila embryo live imaging (upright) can be
> performed as per need?
>
> I would appreciate your valuable inputs!
>
> Warm regards,
>
> Sivaram.
> --
> Sivaram Mylavarapu.
>
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