Posted by
Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583144.html
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Hi Sivaram,
You might also consider purchasing two separate systems, a spinning disc
system for your sensitive imaging, as described, and a build-it-yourself
SPIM system for the live embryos. A PhD student here is planning to
assemble of these "suitcase SPIMs" as described here:
http://openspim.org/Welcome_to_the_OpenSPIM_Wiki. You have to buy (mostly)
or make the components, but from the prices listed, it seems quite cheap
and there are detailed instructions. If you your someone in your lab is
keen, it'd be a fun project.
just a thought...
cheers,
Rosemary
Dr Rosemary White
CSIRO Black Mountain
GPO Box 1600
Canberra, ACT 2601
Australia
T 61 2 6246 5475
E
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On 17/12/14 12:17 PM, "sivaram mylavarapu" <
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>
>Dear colleagues,
>
>We are planning on acquiring a high-speed, high-sensitivity confocal
>fluorescence imaging station for meeting two simultaneous needs: 1) high
>speed for live cell imaging and 2) very high sensitivity to be able to
>capture very low light images. We have been seriously considering a
>spinning disc confocal with an EMCCD camera attachment to meet both these
>needs. We would of course include halogen illumination for routine
>widefield fluorescence imaging.
>
>I wanted to request your opinions on the following:
>
>1) Which is the best microscope in the market for these needs? I have used
>a Zeiss spinning disc in the past, and it appears Leica has an instrument
>too - both use Yokogawa spinning discs. Nikon sells their system with an
>Andor spinning disc.
>
>2) Which is the best EMCCD camera for such a system?
>
>3) Can I combine the above features to be supplied with a dual inverted
>and
>upright microscope so that both high resolution live cell (inverted) and C
>elegans/ Zebrafish/ Drosophila embryo live imaging (upright) can be
>performed as per need?
>
>I would appreciate your valuable inputs!
>
>Warm regards,
>
>Sivaram.
>--
>Sivaram Mylavarapu.
>
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