Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583145.html
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Hi Sivaram,
Consider sCMOS instead of EMCCD. PCO may have a small hardware edge over
Andor and Hamamatsu when using the 4.2 sensor. My Hamamatsu FLASH4
system (MetaMorph 7.8.x) has flaky crashing issues - I will not be
surprised if this is due to the D-CAM driver being poorly written (and
suspect PCO is far better).
Consider the spidery platform on the right side of the instrument
pictured near the bottom of
http://www.biovis.com/diskovery.htmAlso consider the less expensive (and does not require lasers) Crisel
X-Light (3rd generation what started out as Atto CARV) and whatever LED
fluorescence illumination the vendor recommends ... 2 X-Lights + 2 LED
fl illuminators will probably cost less than a Yokogawa+laser stack.
http://www.biovis.com/x-light.htm//
"We would of course include halogen illumination for routine widefield
fluorescence imaging."
Really?
//
spinning disks are so 1996 (maybe 1997 - first gen Yokogawa CSU-10 in
Shinya Inoue's lab, MBL, see for a 12512322 later publication). At least
Olympus FV-OSR and Zeiss AiryScan are pushing point scanning confocals
(and then there is STED - too bad Leica has crappy print ads for it that
leave the side by side images looking pretty much identical).
For speed and resolution (better than any confocal, or FV-OSR or
AiryScan), email or talk with Hari about iSIM:
*Instant* super-resolution imaging in live cells and embryos via analog
image processing. <
http://www.ncbi.nlm.nih.gov/pubmed/24097271>
*York* AG, Chandris P, Nogare DD, Head J, Wawrzusin P, Fischer RS,
Chitnis A, *Shroff*H.
Nat Methods. 2013; 10: 1122-6. doi: 10.1038/nmeth.2687. Epub 2013 Oct 6.
PMID:
24097271
//
Speaking of lasers - only two weeks left before
http://www.wickedlasers.com/ stops selling >5 mW lasers in the USA ...
reminder: do not look at laser with remaining eye.
They apparently will still sell Torch lamp to burn things
http://www.wickedlasers.com/torchEnjoy,
George
On 12/16/2014 7:17 PM, sivaram mylavarapu wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Dear colleagues,
>
> We are planning on acquiring a high-speed, high-sensitivity confocal
> fluorescence imaging station for meeting two simultaneous needs: 1) high
> speed for live cell imaging and 2) very high sensitivity to be able to
> capture very low light images. We have been seriously considering a
> spinning disc confocal with an EMCCD camera attachment to meet both these
> needs. We would of course include halogen illumination for routine
> widefield fluorescence imaging.
>
> I wanted to request your opinions on the following:
>
> 1) Which is the best microscope in the market for these needs? I have used
> a Zeiss spinning disc in the past, and it appears Leica has an instrument
> too - both use Yokogawa spinning discs. Nikon sells their system with an
> Andor spinning disc.
>
> 2) Which is the best EMCCD camera for such a system?
>
> 3) Can I combine the above features to be supplied with a dual inverted and
> upright microscope so that both high resolution live cell (inverted) and C
> elegans/ Zebrafish/ Drosophila embryo live imaging (upright) can be
> performed as per need?
>
> I would appreciate your valuable inputs!
>
> Warm regards,
>
> Sivaram.
>
--
George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales
http://works.bepress.com/gmcnamara/42