Re: High speed spinning disc confocal with EMCCD camera (commercial post)

Posted by Philippe clemenceau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583147.html

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COMMERCIAL POST :

For the community using spinning disk confocal,  I wanted to communicate that there is now an even faster option ( up to several KHz frame rate)  for truly high speed spinning disk confocal , using a high speed , cooled intensified CMOS camera.

We just launched the HICAM FLUO from Lambert Instruments which outputs 1 Mpixels at 1 KHz with cooled Gen 3 intensifier.

Please check http://www.lambertinstruments.com/hicam-fluo <http://www.lambertinstruments.com/hicam-fluo>

This would be interesting for researchers who need to observe very fast phenomenon (ms scale) or perform extremely fast 3D scanning .

Kind regards,

Philippe Clémenceau,
Co-Founder, MS in Optical Science
Axiom Optics
 
 
1 Broadway, 14th floor
Cambridge, MA 02142, USA
 
Phone:  (617) 401 2198
Mobile: (310) 597 1347

Check out our product line at www.axiomoptics.com <http://www.axiomoptics.com/>   :

-Scientific Imaging (CCD, EMCCD, SCMOS, SWIR, CMOS, Intensified )
-Laser Beam profilers (UV , NIR,  IR)
-Advanced Light Microscopy  ( Fluorescence Lifetime, Phase Imaging  & Adaptive Optics)

> On Dec 17, 2014, at 4:24 AM, Andreas Bruckbauer <[hidden email]> wrote:
>
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>
>
> If bleaching and photo damage is an issue (as usually with live cell imaging), check out this high tech solution by the Betzig group:
>
> http://www.sciencemag.org/content/346/6208/1257998.full
> it should be commercially available from 3i
> https://www.intelligent-imaging.com/systems.php#lattice
>
>
> best wishes
>
> Andreas
>
>
>
>
> -----Original Message-----
> From: sivaram mylavarapu <[hidden email]>
> To: CONFOCALMICROSCOPY <[hidden email]>
> Sent: Wed, 17 Dec 2014 1:32
> Subject: High speed spinning disc confocal with EMCCD camera
>
>
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> To join, leave or search the confocal microscopy listserv, go to:
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> Post images on http://www.imgur.com and include the link in your posting.
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>
> Dear colleagues,
>
> We are planning on acquiring a high-speed, high-sensitivity confocal
> fluorescence imaging station for meeting two simultaneous needs: 1) high
> speed for live cell imaging and 2) very high sensitivity to be able to
> capture very low light images. We have been seriously considering a
> spinning disc confocal with an EMCCD camera attachment to meet both these
> needs. We would of course include halogen illumination for routine
> widefield fluorescence imaging.
>
> I wanted to request your opinions on the following:
>
> 1) Which is the best microscope in the market for these needs? I have used
> a Zeiss spinning disc in the past, and it appears Leica has an instrument
> too - both use Yokogawa spinning discs. Nikon sells their system with an
> Andor spinning disc.
>
> 2) Which is the best EMCCD camera for such a system?
>
> 3) Can I combine the above features to be supplied with a dual inverted and
> upright microscope so that both high resolution live cell (inverted) and C
> elegans/ Zebrafish/ Drosophila embryo live imaging (upright) can be
> performed as per need?
>
> I would appreciate your valuable inputs!
>
> Warm regards,
>
> Sivaram.
> --
> Sivaram Mylavarapu.
> [hidden email]
>
>
>
>