Posted by
Zdenek Svindrych-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583172.html
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Hi Sivaram,
I have two additional short comments:
1) Andor uses Yokogawa spinning discs as well.
2) You may also consider structured illumination-based optical sectioning
microscopes. It's nothing to do with superresolution but the sectioning can
in fact be better than with a confocal (
http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-20-22-24585). Old Zeiss ApoTome worked that way. Now
there are better units out there, such as Zeiss VivaTome or Andor Revolution
DSD, that don't do any complicated image processing and try to capture all
the fluorescence coming from your sample (to be fair, there is some
subtraction of fluorescence images, which worsens the SNR somewhat).
I have tried the Andor unit (no commercial interest), they sell it as a
'budget confocal', but I was quite impressed with the results... Also it's
pretty compact and uses arc lamp (or LED) illumination.
Best, zdenek
---------- Původní zpráva ----------
Od: sivaram mylavarapu <
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Komu:
[hidden email]
Datum: 16. 12. 2014 20:33:21
Předmět: High speed spinning disc confocal with EMCCD camera
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Dear colleagues,
We are planning on acquiring a high-speed, high-sensitivity confocal
fluorescence imaging station for meeting two simultaneous needs: 1) high
speed for live cell imaging and 2) very high sensitivity to be able to
capture very low light images. We have been seriously considering a
spinning disc confocal with an EMCCD camera attachment to meet both these
needs. We would of course include halogen illumination for routine
widefield fluorescence imaging.
I wanted to request your opinions on the following:
1) Which is the best microscope in the market for these needs? I have used
a Zeiss spinning disc in the past, and it appears Leica has an instrument
too - both use Yokogawa spinning discs. Nikon sells their system with an
Andor spinning disc.
2) Which is the best EMCCD camera for such a system?
3) Can I combine the above features to be supplied with a dual inverted and
upright microscope so that both high resolution live cell (inverted) and C
elegans/ Zebrafish/ Drosophila embryo live imaging (upright) can be
performed as per need?
I would appreciate your valuable inputs!
Warm regards,
Sivaram.
--
Sivaram Mylavarapu.
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