http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583205.html
light is detected/second. More beams will produce more data/second.
saturation. But the quality of the data will vary between techniques.
I would bet on light sheet/SPIM. Damage only in the illuminated plane
>Hi all,
>
>Does anyone think it would be possible to tabulate a 'speed limit' for the
>various options discussed? I know it sounds near impossible to come up
>with a standard basis for comparison, but let's say something
>approximating a 512x512 acquisition either fixed or or a volume that
>includes 10 z steps (e.g., using a piezo stage when relevant). It would
>be great to have an order of magnitude idea how to compare technologies
>like a resonant scanner, Optera-type swept field scanner, spinning disc,
>VCS super-spinning disc or light sheet instrument when FPS is a major
>priority and excitation light is not limiting. Maybe we could crowdsource
>it from what users actually get in practice.
>
>All the best,
>
>
>Tim
>
>Timothy Feinstein, Ph.D. | Manager, Core for
>Confocal Microscopy and Quantitative Imaging
>333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
>Phone: 616-234-5819 | Email:
[hidden email]
>
>
>
>
>
>
>
>On 12/30/14, 2:36 AM, "Andrea Latini" <
[hidden email]> wrote:
>
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>
http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOL1R>>ax-qpJw&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocalmic
>>roscopy
>>Post images on
>>
http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOLwF>>bleD9dw&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
>>posting.
>>*****
>>
>>Dear Andrew,
>>the VCS (Video Confocal Super Resolution), module is an X-Light Spinning
>>disk system
>>add-on.
>>the disk is out of the optical path when in VCS mode (i.e. 'bypass' mode).
>>basically, it's a new implementation of structured illumination
>>technology aimed to fast
>>image acquisition with no resolution limitations that are spinning disk
>>related.
>>
>>I'll be pleased to discuss more, please get in touch.
>>
>>Regards.
>>
>>Andrea
>>
[hidden email]
>>
>>
>>On Mon, 29 Dec 2014 16:58:15 -0500, Andrew York
>><
[hidden email]> wrote:
>>
>>>*****
>>>To join, leave or search the confocal microscopy listserv, go to:
>>>
http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOL1>>>Rax-qpJw&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocalm
>>>icroscopy
>>>Post images on
>>>
http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOLw>>>FbleD9dw&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
>>>posting.
>>>*****
>>>
>>> Is there information available about this product? Is this an
>>>implementation of Enderlein's spinning disk paper? Also, 80 nm seems...
>>>optimistic? Is this with very short wavelength light, or just a slightly
>>>different definition of resolution than I'm used to?
>>>
>>>On Mon, Dec 29, 2014 at 4:10 PM, Andrea Latini <
[hidden email]>
>>>wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>
>>>>
http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOL>>>>1Rax-qpJw&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfoca
>>>>lmicroscopy
>>>> Post images on
>>>>
http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOL>>>>wFbleD9dw&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
> >>>posting.
>>>> *****
>>>>
>>>> - commercial response
>>>>
>>>> thanks for reporting your experience with our Confocals Marco.
>>>>
>>>> the new Video Super Resolution module for XLight allows for 50ms
>>>>exposure
>>>> time and <1
>>>> second, 80nm spatial resolution; this is possible with large Cuda
>>>> programming we've been
>>>> developing during past months and introduced @SfN 2014 as a product.
>>>>
>>>> soon on our website and in your Lab, hopefully!
>>>>
>>>> Cheers.
>>>>
>>>> Andrea
>>>>
>>>> CrestOptics
>>>>
[hidden email]
>>>>
NEW! NEW! AND DIFFERENT Cell (when I remember to turn it on!) 1-604-989-6146