Re: High speed spinning disc confocal with EMCCD camera - commercial response

Posted by Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583206.html

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Multi-beam multiphoton (eg LaVision Biotec) also limits bleaching to the focal plane and has the advantage over spinning disk confocal that there is no cross-talk.  No commercial association, but I do know a very satisfied user.

                                Guy

Guy Cox, Honorary Associate Professor
School of Medical Sciences

Australian Centre for Microscopy and Microanalysis,
Madsen, F09, University of Sydney, NSW 2006


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of James Pawley
Sent: Sunday, 4 January 2015 11:47 AM
To: [hidden email]
Subject: Re: High speed spinning disc confocal with EMCCD camera - commercial response

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Details aside, data rate will always be proportional to how much light is detected/second. More beams will produce more data/second.
Single beam instruments really can't compete because they intensity in a focused confocal spot is already close to singlet-state saturation. But the quality of the data will vary between techniques.
What do you "need to see"?.

I would bet on light sheet/SPIM. Damage only in the illuminated plane and simple optics to a (effective) high-QE EM-CCD or sCMOS camera.

JP

>Hi all,
>
>Does anyone think it would be possible to tabulate a 'speed limit' for
>the various options discussed?  I know it sounds near impossible to
>come up with a standard basis for comparison, but let's say something
>approximating a 512x512 acquisition either fixed or or a volume that
>includes 10 z steps (e.g., using a piezo stage when relevant).  It
>would be great to have an order of magnitude idea how to compare
>technologies like a resonant scanner, Optera-type swept field scanner,
>spinning disc, VCS super-spinning disc or light sheet instrument when
>FPS is a major priority and excitation light is not limiting.  Maybe we
>could crowdsource it from what users actually get in practice.
>
>All the best,
>
>
>Tim
>
>Timothy Feinstein, Ph.D. | Manager, Core for Confocal Microscopy and
>Quantitative Imaging
>333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
>Phone: [phone] | Email: [hidden email]
>
>
>
>
>
>
>
>On 12/30/14, 2:36 AM, "Andrea Latini" <[hidden email]> wrote:
>
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>>OL1R
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>>lmic
>>roscopy
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>>your posting.
>>*****
>>
>>Dear Andrew,
>>the VCS (Video Confocal Super Resolution), module is an X-Light
>>Spinning disk system add-on.
>>the disk is out of the optical path when in VCS mode (i.e. 'bypass' mode).
>>basically, it's a new implementation of structured illumination
>>technology aimed to fast image acquisition with no resolution
>>limitations that are spinning disk related.
>>
>>I'll be pleased to discuss more, please get in touch.
>>
>>Regards.
>>
>>Andrea
>>[hidden email]
>>
>>
>>On Mon, 29 Dec 2014 16:58:15 -0500, Andrew York
>><[hidden email]> wrote:
>>
>>>*****
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>>>icroscopy
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>>>in your posting.
>>>*****
>>>
>>>  Is there information available about this product? Is this an
>>>implementation of Enderlein's spinning disk paper? Also, 80 nm seems...
>>>optimistic? Is this with very short wavelength light, or just a
>>>slightly different definition of resolution than I'm used to?
>>>
>>>On Mon, Dec 29, 2014 at 4:10 PM, Andrea Latini <[hidden email]>
>>>wrote:
>>>
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>>>>foca
>>>>lmicroscopy
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>>>>in your
>  >>>posting.
>>>>  *****
>>>>
>>>>  - commercial response
>>>>
>>>>  thanks for reporting your experience with our Confocals Marco.
>>>>
>>>>  the new Video Super Resolution module for XLight allows for 50ms
>>>>exposure
>>>>  time and <1
>>>>  second, 80nm spatial resolution; this is possible with large Cuda
>>>>  programming we've been
>>>>  developing during past months and introduced @SfN 2014 as a product.
>>>>
>>>>  soon on our website and in your Lab, hopefully!
>>>>
>>>>  Cheers.
>>>>
>>>>  Andrea
>>>>
>>>>  CrestOptics
>>>>  [hidden email]
>>>>

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