Re: High speed spinning disc confocal with EMCCD camera - commercial response

Posted by sivaram mylavarapu on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583209.html

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Dear colleagues,

Thank you all for your inputs over the past few weeks to my original query
regarding combinations of high-speed microscopes with high sensitivity
cameras for live cell time lapse imaging - all are highly appreciated. The
compilation suggested by Tim Feinstein here would serve as an extremely
useful source for end users including me. If the list will indeed be
compiled and could also include cameras based on a hierarchy of sensitivity
(EMCCD, sCMOS, others) to go with the illumination technologies, it would
be of great help.

Warm regards for the New Year!

Sivaram.

On Sun, Jan 4, 2015 at 3:42 AM, Feinstein, Timothy <
[hidden email]> wrote:

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> *****
>
> Hi all,
>
> Does anyone think it would be possible to tabulate a ¹speed limit' for the
> various options discussed?  I know it sounds near impossible to come up
> with a standard basis for comparison, but let¹s say something
> approximating a 512x512 acquisition either fixed or or a volume that
> includes 10 z steps (e.g., using a piezo stage when relevant).  It would
> be great to have an order of magnitude idea how to compare technologies
> like a resonant scanner, Optera-type swept field scanner, spinning disc,
> VCS super-spinning disc or light sheet instrument when FPS is a major
> priority and excitation light is not limiting.  Maybe we could crowdsource
> it from what users actually get in practice.
>
> All the best,
>
>
> Tim
>
> Timothy Feinstein, Ph.D. | Manager, Core for
> Confocal Microscopy and Quantitative Imaging
> 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
> Phone: [phone] | Email: [hidden email]
>
>
>
>
>
>
>
> On 12/30/14, 2:36 AM, "Andrea Latini" <[hidden email]> wrote:
>
> >*****
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> >ax-qpJw&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocalmic
> >roscopy
> >Post images on
> >
> http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOLwF
> >bleD9dw&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
> >posting.
> >*****
> >
> >Dear Andrew,
> >the VCS (Video Confocal Super Resolution), module is an X-Light Spinning
> >disk system
> >add-on.
> >the disk is out of the optical path when in VCS mode (i.e. 'bypass' mode).
> >basically, it's a new implementation of structured illumination
> >technology aimed to fast
> >image acquisition with no resolution limitations that are spinning disk
> >related.
> >
> >I'll be pleased to discuss more, please get in touch.
> >
> >Regards.
> >
> >Andrea
> >[hidden email]
> >
> >
> >On Mon, 29 Dec 2014 16:58:15 -0500, Andrew York
> ><[hidden email]> wrote:
> >
> >>*****
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> >>Rax-qpJw&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocalm
> >>icroscopy
> >>Post images on
> >>
> http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOLw
> >>FbleD9dw&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
> >>posting.
> >>*****
> >>
> >> Is there information available about this product? Is this an
> >>implementation of Enderlein's spinning disk paper? Also, 80 nm seems...
> >>optimistic? Is this with very short wavelength light, or just a slightly
> >>different definition of resolution than I'm used to?
> >>
> >>On Mon, Dec 29, 2014 at 4:10 PM, Andrea Latini <[hidden email]>
> >>wrote:
> >>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>>
> >>>
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> >>>1Rax-qpJw&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfoca
> >>>lmicroscopy
> >>> Post images on
> >>>
> http://scanmail.trustwave.com/?c=129&d=09ai1Hwf389A_JSBGnaEcBqKN1nFKNqOL
> >>>wFbleD9dw&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
> >>>posting.
> >>> *****
> >>>
> >>> - commercial response
> >>>
> >>> thanks for reporting your experience with our Confocals Marco.
> >>>
> >>> the new Video Super Resolution module for XLight allows for 50ms
> >>>exposure
> >>> time and <1
> >>> second, 80nm spatial resolution; this is possible with large Cuda
> >>> programming we've been
> >>> developing during past months and introduced @SfN 2014 as a product.
> >>>
> >>> soon on our website and in your Lab, hopefully!
> >>>
> >>> Cheers.
> >>>
> >>> Andrea
> >>>
> >>> CrestOptics
> >>> [hidden email]
> >>>
>

--
Sivaram Mylavarapu.
[hidden email]