Posted by
Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583217.html
Michael,
Both you and I have been telling users this for years. Likewise, if they want to image a mitochondrion, they gain nothing by going beyond Nyquist, yet they still zoom it up to 1024 x 768. These people are supposed to be scientists - but are they? A microscope is a scientific instrument, not a black box.
Guy
Guy Cox, Honorary Associate Professor
School of Medical Sciences
Australian Centre for Microscopy and Microanalysis,
Madsen, F09, University of Sydney, NSW 2006
-----Original Message-----
From: Cammer, Michael [mailto:
[hidden email]]
Sent: Tuesday, 6 January 2015 2:57 AM
To: Guy Cox
Subject: RE: High speed spinning disc confocal with EMCCD camera - commercial response
I constantly tell people doing live imaging with the multiphoton who want to track cells to turn down the excitation and do running averages or other noise filtering later. I show them examples but when I leave the room they invariably crank up the light because they want to see a perfect low noise high contrast image right now and then they are disappointed when the experiment doesn't work either because the sample bleaches or the cells stop moving.
=========================================================================
Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 Temporary location: SK2-7
http://ocs.med.nyu.edu/microscopy &
http://microscopynotes.com/-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Guy Cox
Sent: Monday, January 05, 2015 10:45 AM
To:
[hidden email]
Subject: Re: High speed spinning disc confocal with EMCCD camera - commercial response
Not being able to saturate excitation is a good thing, not a bad thing! I am perpetually telling people to reduce their excitation to 50% and check that their excitation decreases by the same amount - if not they are saturating (and destroying) their fluorochrome. Nobody ever follows my advice - they just want a bright (and usually saturated and therefore uninformative) image.
Guy
Guy Cox, Honorary Associate Professor
School of Medical Sciences
Australian Centre for Microscopy and Microanalysis, Madsen, F09, University of Sydney, NSW 2006