http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583219.html
p.s.
> Michael,
>
> Both you and I have been telling users this for years. Likewise, if they want to image a mitochondrion, they gain nothing by going beyond Nyquist, yet they still zoom it up to 1024 x 768. These people are supposed to be scientists - but are they? A microscope is a scientific instrument, not a black box.
>
> Guy
>
> Guy Cox, Honorary Associate Professor
> School of Medical Sciences
>
> Australian Centre for Microscopy and Microanalysis,
> Madsen, F09, University of Sydney, NSW 2006
>
>
> -----Original Message-----
> From: Cammer, Michael [mailto:
[hidden email]]
> Sent: Tuesday, 6 January 2015 2:57 AM
> To: Guy Cox
> Subject: RE: High speed spinning disc confocal with EMCCD camera - commercial response
>
> I constantly tell people doing live imaging with the multiphoton who want to track cells to turn down the excitation and do running averages or other noise filtering later. I show them examples but when I leave the room they invariably crank up the light because they want to see a perfect low noise high contrast image right now and then they are disappointed when the experiment doesn't work either because the sample bleaches or the cells stop moving.
> =========================================================================
> Michael Cammer, Microscopy Core& Skirball Institute, NYU Langone Medical Center
> Cell: 914-309-3270 Temporary location: SK2-7
>
http://ocs.med.nyu.edu/microscopy& http://microscopynotes.com/>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Guy Cox
> Sent: Monday, January 05, 2015 10:45 AM
> To:
[hidden email]
> Subject: Re: High speed spinning disc confocal with EMCCD camera - commercial response
>
>
> Not being able to saturate excitation is a good thing, not a bad thing! I am perpetually telling people to reduce their excitation to 50% and check that their excitation decreases by the same amount - if not they are saturating (and destroying) their fluorochrome. Nobody ever follows my advice - they just want a bright (and usually saturated and therefore uninformative) image.
>
> Guy
>
>
> Guy Cox, Honorary Associate Professor
> School of Medical Sciences
>
> Australian Centre for Microscopy and Microanalysis, Madsen, F09, University of Sydney, NSW 2006
>
>
>
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center