http://confocal-microscopy-list.275.s1.nabble.com/High-speed-spinning-disc-confocal-with-EMCCD-camera-tp7583142p7583222.html
You probably mean "diffraction" rather than "Nyquist". The Nyquist limit
not. Guy's point is that one only needs to sample densely enough to
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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>
> Hi Guy,
>
> Nyquist is dead.
>
> For mitochondria way beyond, see Shim et al 2012,
>
http://www.ncbi.nlm.nih.gov/pubmed/22891300>
http://www.pnas.org/content/109/35/13978.long (open access)
>
> also several papers from Hell and colleagues, such as;
>
>
http://www.ncbi.nlm.nih.gov/pubmed/20205711>
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838807/pdf/1757-5036-3-4.pdf>
> and
> 30 nm isotropic resolution (isoSTED)
>
http://www.ncbi.nlm.nih.gov/pubmed/19459703>
> George
> p.s.
http://en.wikipedia.org/wiki/Harry_Nyquist> *Harry Nyquist* (/né/ Harry Theodor Nyqvist; / <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English>ˈ <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>n <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>aɪ <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>k <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>w <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>ɪ <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>s <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>t <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English#Key>/ <
>
http://en.wikipedia.org/wiki/Help:IPA_for_English>, Swedish: [nʏːkvɪst] <
>
http://en.wikipedia.org/wiki/Help:IPA_for_Swedish_and_Norwegian>;
> February 7, 1889 – April 4, 1976)
>
> On 1/5/2015 10:10 AM, Guy Cox wrote:
>
>> Michael,
>>
>> Both you and I have been telling users this for years.
>> Likewise, if they want to image a mitochondrion, they gain nothing by going
>> beyond Nyquist, yet they still zoom it up to 1024 x 768. These people are
>> supposed to be scientists - but are they? A microscope is a scientific
>> instrument, not a black box.
>>
>> Guy
>>
>> Guy Cox, Honorary Associate Professor
>> School of Medical Sciences
>>
>> Australian Centre for Microscopy and Microanalysis,
>> Madsen, F09, University of Sydney, NSW 2006
>>
>>
>> -----Original Message-----
>> From: Cammer, Michael [mailto:
[hidden email]]
>> Sent: Tuesday, 6 January 2015 2:57 AM
>> To: Guy Cox
>> Subject: RE: High speed spinning disc confocal with EMCCD camera -
>> commercial response
>>
>> I constantly tell people doing live imaging with the multiphoton who want
>> to track cells to turn down the excitation and do running averages or other
>> noise filtering later. I show them examples but when I leave the room they
>> invariably crank up the light because they want to see a perfect low noise
>> high contrast image right now and then they are disappointed when the
>> experiment doesn't work either because the sample bleaches or the cells
>> stop moving.
>> =========================================================================
>> Michael Cammer, Microscopy Core& Skirball Institute, NYU Langone
>> Medical Center
>> Cell: 914-309-3270 Temporary location:
>> SK2-7
>>
http://ocs.med.nyu.edu/microscopy&>>
http://microscopynotes.com/>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:
[hidden email]]
>> On Behalf Of Guy Cox
>> Sent: Monday, January 05, 2015 10:45 AM
>> To:
[hidden email]
>> Subject: Re: High speed spinning disc confocal with EMCCD camera -
>> commercial response
>>
>>
>> Not being able to saturate excitation is a good thing, not a bad thing!
>> I am perpetually telling people to reduce their excitation to 50% and check
>> that their excitation decreases by the same amount - if not they are
>> saturating (and destroying) their fluorochrome. Nobody ever follows my
>> advice - they just want a bright (and usually saturated and therefore
>> uninformative) image.
>>
>> Guy
>>
>>
>> Guy Cox, Honorary Associate Professor
>> School of Medical Sciences
>>
>> Australian Centre for Microscopy and Microanalysis, Madsen, F09,
>> University of Sydney, NSW 2006
>>
>>
>>
>>
>
>
> --
>
>
>
> George McNamara, Ph.D.
> Single Cells Analyst
> L.J.N. Cooper Lab
> University of Texas M.D. Anderson Cancer Center
> Houston, TX 77054
> Tattletales
http://works.bepress.com/gmcnamara/42>