http://confocal-microscopy-list.275.s1.nabble.com/sCMOS-salt-n-pepper-issues-tp7583323p7583336.html
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> Can you share raw data? Also, how are you doing your illumination?
> On Jan 20, 2015 11:19 PM, "Neil Anthony" <
[hidden email]> wrote:
>
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>> Hi all, I hope the science/optics/hardware/software/students/post-docs/
>> reviewers/managers/next-door-neighbors and FedEx are treating you well.
>>
>> We have a couple of issues with a recently installed Hamamatsu Flash 4.0
>> sCMOS camera and I was wondering if anybody has had any similar
>> experiences, both in terms of feedback on what is expected from sCMOS
>> hardware and what can be done to rectify any issues.
>>
>> Here's what we're seeing:
>> Using a Hamamatsu Flash 4.0 camera link sCMOS (firmware v3.00A) on a
>> spinning disk and acquiring data using Perkin Elmer Volocity 6.3 we're
>> having three issues regarding hot and cold pixels. So far we have tried 4
>> different sCMOS cameras (kindly supplied/tested by Hamamatsu and Perkin
>> Elmer), to double check the subtleties, but the results are quite similar
>>
>> On the first camera we saw a bright cluster of 4 pixels that are between 2
>> and 10x greater than the surrounding pixels across nearly all imaging
>> conditions (not seen in the other three cameras). Are these bright pixel
>> clusters something that others have seen much? This issue is seen in both
>> Volocity and ExCap/HDImage, but was not seen on the test sCMOS camera used
>> to cross check. I also see 'ghost' clusters that look similar but only
>> seem to last for one frame... Again, is that something seen in sCMOS
>> cameras in general?
>>
>> Salt:
>> In images with low signal and longer exposure times we see speckled bight
>> pixels using both Volocity and ExCap/HDImage, with an intensity approx
>> twice that of the background signal. Exposure times ~secs; low signal <1000
>> counts per pixel. These speckled hot spots were seen with all sCMOS
>> cameras to some degree.
>> Pepper:
>> In almost the inverse situation, where we have higher signals with lower
>> exposure times were also seen on all sCMOS cameras (the first camera with
>> the clear hotspot had very bad 'peppering' compared to the others).
>>
>> I know that the pco cameras used on the Deltavision OMX have reference
>> images applied to reduce the affects of these artifacts, and I was
>> wondering if that's something that can be applied post-acquisition. Any
>> thoughts and/or feedback on what's normal for an sCMOS, and how to get the
>> best out of these cameras would be much appreciated. sCMOS cameras
>> certainly are fast and have a great signal to noise, so am I expecting too
>> much from this generation of hardware?
>>
>> Thanks
>> Neil
>>
>>
>> A couple of images to compare salt'n'pepper pixels seen on three cameras
>> are here:
>>
https://drive.google.com/file/d/0BzfJAGNfrgieQ21abmVPbFBqY0k/>> view?usp=sharing
>>
https://drive.google.com/file/d/0BzfJAGNfrgieVjViTWNoeExpdDQ/>> view?usp=sharing
>>