Re: darkfield references

Posted by John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/darkfield-references-tp7583371p7583372.html

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Jeff,

See this paper:

Vainrub, A., O. Pustovyy, and V. Vodyanoy, Resolution of 90 nm (lambda/5) in an optical transmission microscope with an annular condenser. Optics Letters, 2006. 31(19): p. 2855-2857.

John Oreopoulos
Staff Scientist
Spectral Applied Research Inc.
A Division of Andor Technology
Richmond Hill, Ontario
Canada
www.spectral.ca



On 2015-01-30, at 5:44 PM, Jeff Spector wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Greetings,
>   Can someone please point me to some references involving the theory
> behind darkfield microscopy? I understand the basic idea, but all I can
> find are different iterations of the basic idea that you block most of the
> light and only image scattered light. I'd like to learn a bit more about
> technical aspects of  darkfield, i.e. what is the smallest object you can
> observe? What role do illumination power and camera exposure play in the
> quality of the final image. What role does specimen thickness/size play in
> the final image and can you discern objects of different size
> etc...
> Any help would be greatly appreciated. Perhaps I simply need to read up on
> scattering theory?
> thanks..
> -jeff