http://confocal-microscopy-list.275.s1.nabble.com/Preamplifier-for-fast-point-scanning-tp7583344p7583381.html
is sufficient for video rate imaging. I don't remember the precise
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>
> Hi Peter,
> I tell you 80 MHz is a *high* frequency. What are you aiming at? 1024 x
> 1024
> pixels at 80 fps? That sounds cool. But how many photons do you think
> you'll
> get in a pixel? I bet mostly zero. Maybe one, from time to time... So you
> may end up with heavy smoothing anyway. If you get whopping 10^9 photons
> per
> second in the bright parts of your image, that translates to a SNR of 3.5
> :-
> ).
>
> I don't want to put you off, just go ahead and try it (and keep us posted).
> Just remember that every piece of cable needs proper impedance matching
> (termination), also the 'speed of light' is somewhat lower in a cable (20
> cm
> / ns), also add some 1 to 5 ns per each comparator or amplifier stage and
> you may end up not knowing which pulse came from which pixel (and of course
> another 3 ns delay due to the lifetime of your fluorescent protein).
>
> Detectors with integrated amplifiers are also available, but it's either
> low
> speed 'intensity based', with some 6 MHz bandwidth, or fast photon counting
> (and these are currently not able to do 10^9 counts per second). I haven't
> seen anything in between that would be suitable for your project. So
> combining fast detector, fast mplifier and fast digitizer is challenging,
> even if you use commercially available building blocks...
>
> I know I didn't help much, but, good luck!
>
> zdenek
>
>
> --
> Zdenek Svindrych, Ph.D.
> W.M. Keck Center for Cellular Imaging (PLSB 003)
> University of Virginia, Charlottesville, USA
>
http://www.kcci.virginia.edu/workshop/index.php>
>
>
> ---------- Původní zpráva ----------
> Od: Peter Rupprecht <
[hidden email]>
> Komu:
[hidden email]
> Datum: 27. 1. 2015 4:41:25
> Předmět: Preamplifier for fast point-scanning
>
> "*****
> To join, leave or search the confocal microscopy listserv, go to:
>
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> *****
>
> Dear list,
>
> I'm looking for preamplifiers for use in a resonant scanning 2P microscope,
> using fluorophors like GCaMP or OGB in vivo. Acquisition will be
> time-locked
> to the 80 MHz laser pulses, so the bandwidth of the PMT preamplifier should
> be at least 80 MHz in order not to smooth adjacent pixels too much.
>
> Right now, I'm using this model, which seems to be used by many others in
> the field (as far as I can see):
>
>
http://www.femto.de/en/products/current-amplifiers/variable-gain-up-to-200-> mhz-dhpca.html
>
> Is there another option which might be better than this standard solution
> for some applications? I'd be glad to hear some opinions on that.
>
> Best regards,
> Peter"
>