Best Sampling for nucleus quantification in wide-field

Posted by c.machu on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-Sampling-for-nucleus-quantification-in-wide-field-tp7583395.html

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Hello everyone !!

I have a very silly question. In our lab we would like to be able quantify
the whole signal intensity within a yeast nucleus. I know how to measure it
easily but I have doubts the Z sampling I am using. Should I sample using a
Nyquist criterion (but counting several time the same molecule in the
nucleus) or should I assume the theoretical Z resolution of my microscope
(knowing my objective specifics) ?

I would personally vote for the second option but I'd be glad to be
corrected if I am wrong.

Thanks a lot in advance for our inputs.

Best, Christophe