Posted by
Michael Giacomelli on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Leica-slide-scanner-calibration-tp7583619p7583625.html
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Hi Brian,
I'm a little surprised that this is an issue, but I think you may be
right. Adjusting the gamma by approximately that amount makes the
images look better. I'm not certain they're correct, but its certainly
much better.
A question though: why is this even a problem? As I understand it,
the Aperio format includes color calibration information from the
scanner (theres an option to apply it in their software), and it will
take the color calibration from my monitor in Windows. So it knows
the gamma of both the display and scanner. Why does it still end up
wrong? Is this a bug in Leica's software or do I not understand
something?
As an aside, I googled this imaging core and Aperio, and it brings up
a lot of papers with badly oversaturated slides, so I'm pretty sure
this isn't just me.
Mike
On Mon, Apr 6, 2015 at 7:18 PM, Armstrong, Brian <
[hidden email]> wrote:
> Hello Mike, I believe that some scanners use a default gamma setting of 0.45 for BF imaging. If this is the case then with the gamma at 1.0 it will appear oversaturated. Take a look at your gamma settings on Aperio Image Scope and see where it is set. Try the gamma at 0.45 and see how it looks. Ask your imaging partner what the gamma setting is on the Aperio scanner.
>
> Cheers,
>
> Brian Armstrong PhD
> Director, Light Microscopy Core
> Beckman Research Institute
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Michael Giacomelli
> Sent: Monday, April 06, 2015 4:02 PM
> To:
[hidden email]
> Subject: Leica slide scanner calibration
>
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> I had some pathology slides scanned at a local imaging core on an Aperio scanner, but the color appears to be quite off. Using the built in correction in the aperio visualization software (which I believe uses the correction information from the scanner), my H&E slides are kind of a hot, oversaturated pink. Disabling it brings it closer to reality, but its still much too saturated. Just to convince myself, I looked side by side between the scanned images and the original slides and used a second monitor. Something is wrong.
>
> I don't think this is my monitor, its an 8 bit IPS panel thats been color calibrated to ~100% sRGB using an X-Rite. Besides giving excellent accuracy in the X-Rite measurements, other images look fine.
>
> My assumption is that it has to be the scanner itself. Has anyone had this problem before? I'm going to go back to the imaging core and try to talk to them about it, but I was hoping to find out a little bit more about the problem so I don't sound clueless when I say that my images look 'funny'.
>
> Mike
>
>
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