PSF asymmetry

Posted by Lathrop, Kira L on
URL: http://confocal-microscopy-list.275.s1.nabble.com/PSF-asymmetry-tp7583710.html

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Dear Christophe,
When I started here I was testing PSFs on one of the scopes and was getting dramatic asymmetry in several objectives. After much hunting and testing it turned out that the objective turret was cross-threaded. You might just try unscrewing your whole objective turret and reseating it.
Best of luck,
Kira

Kira L. Lathrop, MAMS
Assistant Professor
Co-Director, OVSRC Imaging Module
University of Pittsburgh
School of Medicine, Department of Ophthalmology
Swanson School of Engineering, Department of Bioengineering
203 Lothrop Street Room 1032
Pittsburgh, PA 15261
[hidden email]
412.647.3492