Posted by
Sarah Richert on
URL: http://confocal-microscopy-list.275.s1.nabble.com/mEos4-photoconversion-tp7583802p7583803.html
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Dear Jordan,
i am working with mEos2 but dont have experience with mEos4, but still i
would like to suggest two things:
1) i have observed photoconversion to some extent by illumination with a
UV-Lamp and a green filter when the lamp is very strong. Is it possible to
identify the cells in brightfield and image in the red channel first to see
whether the 488nm illumination already photoconverts?
2) regarding the photoconversion for me it works better to have a high power
output and less repetitions, but i dont know if thats genereally the case.
In the beginning i tried to photoconvert the total green signal to red
aswell, but realised that i dont yield a strong signal at the end anymore
most likely due to bleaching caused by high energy input. Additionally, at
least my cells, would not survive this procedure. Now i´m working with
approximately 50/50 green to red signal.
Best,
Sarah