Re: mEos4 photoconversion

Posted by Jordan Becker on
URL: http://confocal-microscopy-list.275.s1.nabble.com/mEos4-photoconversion-tp7583802p7583809.html

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Jeff,

It's great to find you on here! I wondered if the LED was sending some
shorter wavelength light that was prematurely switching some to red. From
what I can find in previous literature, low power on the 488 laser and high
on the 561 is a good starting place for visualizing. I think if I can
decrease the premature red signal, the photoconversion ratio should improve
accordingly.

Thanks for the help Sarah and I'll hope to see you around next week Jeff.

Best,
Jordan