Re: mEos4 photoconversion

Posted by Stuckey, Jeff on
URL: http://confocal-microscopy-list.275.s1.nabble.com/mEos4-photoconversion-tp7583802p7583810.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jordan

We weren't using the transmitted LED the other day.  For visualizing with transmitted light we could put a filter in the path that would block the absorption wavelengths for eos if that was a concern.

Best

Jeff

Sent from my iPhone

> On May 30, 2015, at 10:15 AM, "Jordan Becker" <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Jeff,
>
> It's great to find you on here! I wondered if the LED was sending some
> shorter wavelength light that was prematurely switching some to red. From
> what I can find in previous literature, low power on the 488 laser and high
> on the 561 is a good starting place for visualizing. I think if I can
> decrease the premature red signal, the photoconversion ratio should improve
> accordingly.
>
> Thanks for the help Sarah and I'll hope to see you around next week Jeff.
>
> Best,
> Jordan