ATTO dyes for live-cell experiments

Posted by Graham Wright-2 on Jun 19, 2015; 8:25am
URL: http://confocal-microscopy-list.275.s1.nabble.com/ATTO-dyes-for-live-cell-experiments-tp7583874.html

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Hello,

I would like to ask for some opinions/feedback from those with experience
of using ATTO488 and ATTO565 for live-cell imaging experiments (conjugated
to peptides), particularly in terms of photostability/bleaching (planned
for WF and spinning disk microscopy).

I heard from someone that the ATTO565 bleaches relatively quickly (not sure
on their imaging parameters), but would like to hear more. Would people
recommend any other dyes in this spectral range (561 nm laser) that they
have used for these sort of experiments?

Thanks in advance,
Graham

--
Graham Wright, PhD
Head, Microscopy Unit

Institute of Medical Biology, A*STAR
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

W:  http://www.a-star.edu.sg/imb/Tech-Platforms/IMB-Microscopy-Unit.aspx