Re: ATTO dyes for live-cell experiments

Posted by lechristophe on Jun 19, 2015; 8:45am
URL: http://confocal-microscopy-list.275.s1.nabble.com/ATTO-dyes-for-live-cell-experiments-tp7583874p7583875.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Graham,

This reference could be useful:

Bosch PJ, Corrêa IR, Sonntag MH, Ibach J, Brunsveld L, Kanger JS &
Subramaniam V (2014) Evaluation of Fluorophores to Label SNAP-Tag Fused
Proteins for Multicolor Single-Molecule Tracking Microscopy in Live
Cells. *Biophys.
J* *107:* 803–814

They have tested 6 green and 16 red dyes for single molecule tracking in
live cells.

Hope this helps,

Christophe




On Fri, Jun 19, 2015 at 10:25 AM, Graham Wright <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> I would like to ask for some opinions/feedback from those with experience
> of using ATTO488 and ATTO565 for live-cell imaging experiments (conjugated
> to peptides), particularly in terms of photostability/bleaching (planned
> for WF and spinning disk microscopy).
>
> I heard from someone that the ATTO565 bleaches relatively quickly (not sure
> on their imaging parameters), but would like to hear more. Would people
> recommend any other dyes in this spectral range (561 nm laser) that they
> have used for these sort of experiments?
>
> Thanks in advance,
> Graham
>
> --
> Graham Wright, PhD
> Head, Microscopy Unit
>
> Institute of Medical Biology, A*STAR
> 8A Biomedical Grove, #06-06 Immunos, Singapore 138648
>
> W:  http://www.a-star.edu.sg/imb/Tech-Platforms/IMB-Microscopy-Unit.aspx
>