Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/7th-Course-on-Optical-Microscopy-Imaging-for-Biosciences-20-24-April-2015-IBMC-Porto-tp7583912p7583942.html
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Your question is fairly vague, but here are some general answers: With 2
photon the important thing is how well the laser is focused. This means
your objective has to be well corrected for the NIR beam from the laser.
The resulting visible fluorescence is directed towards wide-aperture PMTs,
which don't care about aberration very much.
For single-photon confocal, you have to get your fluorescence through the
confocal pinhole, which will be *very* picky about aberration. Since you
are trying to get the light through a very small aperture any aberration
will cause signal loss.
Craig
On Mon, Jun 29, 2015 at 4:04 PM, Vitaly Boyko <
[hidden email]>
wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
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> Dear List,
> I would greatly appreciate if someone could shed light on the extent of
> lateral and axial chromatic aberrations correction, and its limitations in
> the VIS portion of spectrum when image has been projected into the
> non-descanned detectors (one and two photon imaging)?
> Many thanks in advance.
> Vitaly
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