Re: Lightsheet z.1 vs ultramicroscope

Posted by Michael Weber-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Lightsheet-z-1-vs-ultramicroscope-tp7583940p7583969.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Vladimir,

LightSheet Z.1 and Ultramicroscope are two very different microscopes. The first could be a replacement for a spinning disc confocal, whereas the latter is closer to a point-scanning confocal. It really depends on what kind of specimen you are using.

The Zeiss system is designed for fast imaging of living samples, multi-view recordings, time-lapse imaging. Optics are optimized for the refractive index of water, the specimen can be heated and supplied with CO2. The sample is hanging from top and can be rotated by 360 deg, therefore it must be mounted in a suitable manner.

The LaVision system is made for fixed, cleared samples. It is not overly fast and the specimen cannot be rotated. However, because of the different design you can use all kinds of solvents, which might be essential for recordings of thick tissues or large specimen.

Best,
Michael


On Jun 29, 2015, at 11:55 PM, Vladimir Ghukasyan <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> We are considering getting a light sheet setup for our core. I had a chance
> to test briefly the Z.1 system from Zeiss and prepare to send some samples
> to Germany to test the Ultramicroscope. Havy any of you worked with either
> of these systems? I would be very thankful for any suggestion/comment
> advice on this matter.
>
> Our typical application is imaging of CLARITY cleared mouse brain samples.
> High resolution may be very beneficial (imaging of axonal tracks) but not
> absolutely necessary at this point. The Z.1 can work with a "CLARITY" long
> working distance objectives, but the immersion media is limited to
> non-organic solvents, so no BAAB/iDISCO, etc. Ultramicroscope as I have
> learned today, can be used with any objective, but I wonder how is
> chromatic aberration correction handled (hope to hear about that from the
> company soon).
>
> Thank you in advance,
> Vladimir

_____________

Michael Weber
Postdoc, Huisken lab
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden
Tel. [phone]

http://www.mpi-cbg.de/huisken