Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Boosting-bright-field-resolution-with-dichroic-filters-tp7583983p7583991.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Dear all,
two questions, first about the formula:
I thought 0.61*lambda/(NA) (Rayleigh) would be the appropriate formula
for self-luminous objects (dark field, fluorescence) while for bright
field the fitting variant of the Abbe formula should be applied:
lambda/NA-obj for central illumination, lambda/2NA for NA-obj = NA cond
and, in general, lambda/(NA-obj + NA cond), provided that NA-obj is
equal or bigger than NA-cond.
It seems though, that others are happy with 1.2*lambda/(NA-obj + NA
cond). Where does the 1.2 (or 2*0.6) in a bright field situation come
from? Would that be the paper by Hopkins and Barham (1950), that Mike
was referring to? Because I have no hope of understanding this paper by
reading through during this life (Lots of formulas my biologist's brain
is not flexible enough to adjust for).
Second:
Is there a good source for diatoms that can be stained with fluorescent
dyes and self-mounted, as George suggested? I have a few diatom slides
for teaching (Pleurosigma), but they came already mounted.
Steffen
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
>
> Marchioninistr. 27
> D-81377 München
> Germany