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>Dear all,
>
>two questions, first about the formula:
>
>I thought 0.61*lambda/(NA) (Rayleigh) would be the appropriate formula
>for self-luminous objects (dark field, fluorescence) while for bright
>field the fitting variant of the Abbe formula should be applied:
>lambda/NA-obj for central illumination, lambda/2NA for NA-obj = NA cond
>and, in general, lambda/(NA-obj + NA cond), provided that NA-obj is
>equal or bigger than NA-cond.
>
>It seems though, that others are happy with 1.2*lambda/(NA-obj + NA
>cond). Where does the 1.2 (or 2*0.6) in a bright field situation come
>from? Would that be the paper by Hopkins and Barham (1950), that Mike
>was referring to? Because I have no hope of understanding this paper by
>reading through during this life (Lots of formulas my biologist's brain
>is not flexible enough to adjust for).
>
>Second:
>Is there a good source for diatoms that can be stained with fluorescent
>dyes and self-mounted, as George suggested? I have a few diatom slides
>for teaching (Pleurosigma), but they came already mounted.
>
>Steffen
>
>>
>>
>> --
>> ------------------------------------------------------------
>> Steffen Dietzel, PD Dr. rer. nat
>> Ludwig-Maximilians-Universität München
>> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
>> Head of light microscopy
>>
>> Marchioninistr. 27
>> D-81377 München
>> Germany