Reporting laser power in publication

Posted by Jolien Tyler-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Reporting-laser-power-in-publication-tp7584120.html

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Hello All,

I have a user who is examining heat shock proteins and, long story really
short, is using our laser scanning confocal to blast ROIs of with 405 nm
laser light and examining hsp response.  I'm trying to determine how best
to report the amount of "blasting" being done to the samples.

I have a power meter that I can place at the sample plane to collect power
in milliwatts accounting for the optical light path/objective.  I know the
pixel size in microns being imaged.  The power meter result is per pixel
since this is a laser scanning confocal, regardless of the size of the
power meter detector (correct?).  Thus, I should be able to simply divide
the power meter result and by pixel area to get mW per square micron
(irradiance).  Or am I totally off-track here?

Thanks,
Jolien



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Jolien Tyler, Ph.D.

University of Colorado
MCD Biology
347 UCB
Boulder, CO 80309
303-492-5955 (office)

http://mcdb.colorado.edu/facilities/lmcf/index.html