Re: Reporting laser power in publication

Posted by Michael Giacomelli on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Reporting-laser-power-in-publication-tp7584120p7584121.html

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Does your system use linear in time scans (e.g. galvos) or sinusoidal
in time scans (resonant scanners)?  Do you block off the flyback?

But yes, if you are scanning perfectly uniformly and are blocking the
flyback time on the scanners, the average power from a meter can be
divided by the scan area to compute the power/area.  If you are not
blocking the flyback or are resonant, you will be off by some,
although perhaps not enough that it matters for your purposes.

Mike

On Fri, Aug 14, 2015 at 1:08 PM, Jolien Tyler <[hidden email]> wrote:

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>
> Hello All,
>
> I have a user who is examining heat shock proteins and, long story really
> short, is using our laser scanning confocal to blast ROIs of with 405 nm
> laser light and examining hsp response.  I'm trying to determine how best
> to report the amount of "blasting" being done to the samples.
>
> I have a power meter that I can place at the sample plane to collect power
> in milliwatts accounting for the optical light path/objective.  I know the
> pixel size in microns being imaged.  The power meter result is per pixel
> since this is a laser scanning confocal, regardless of the size of the
> power meter detector (correct?).  Thus, I should be able to simply divide
> the power meter result and by pixel area to get mW per square micron
> (irradiance).  Or am I totally off-track here?
>
> Thanks,
> Jolien
>
>
>
> ---------------------------------------------
> Jolien Tyler, Ph.D.
>
> University of Colorado
> MCD Biology
> 347 UCB
> Boulder, CO 80309
> 303-492-5955 (office)
>
> http://mcdb.colorado.edu/facilities/lmcf/index.html