Re: Reporting laser power in publication

Posted by Sivaguru, Mayandi on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Reporting-laser-power-in-publication-tp7584120p7584122.html

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Jolien, when you use the power meter make sure, you have tuned to the
right wavelength on the power meter and it has sensitivity in that range
and no polarizers/prisms in the path. Also you can use the digital zoom
to increase to the max, i.e. 40x in the scan area so the galvos are
parked not scanning while you make your measurement.

On 8/14/2015 12:08 PM, Jolien Tyler wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello All,
>
> I have a user who is examining heat shock proteins and, long story really
> short, is using our laser scanning confocal to blast ROIs of with 405 nm
> laser light and examining hsp response.  I'm trying to determine how best
> to report the amount of "blasting" being done to the samples.
>
> I have a power meter that I can place at the sample plane to collect power
> in milliwatts accounting for the optical light path/objective.  I know the
> pixel size in microns being imaged.  The power meter result is per pixel
> since this is a laser scanning confocal, regardless of the size of the
> power meter detector (correct?).  Thus, I should be able to simply divide
> the power meter result and by pixel area to get mW per square micron
> (irradiance).  Or am I totally off-track here?
>
> Thanks,
> Jolien
>
>
>
> ---------------------------------------------
> Jolien Tyler, Ph.D.
>
> University of Colorado
> MCD Biology
> 347 UCB
> Boulder, CO 80309
> 303-492-5955 (office)
>
> http://mcdb.colorado.edu/facilities/lmcf/index.html

--
Mayandi Sivaguru PhD (IND), PhD (JPN)
Assistant Director of Core Facilities
University of Illinois at Urbana-Champaign
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