detecting apoptosis in one cell type in coculture

Posted by Leoncio Vergara-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/detecting-apoptosis-in-one-cell-type-in-coculture-tp7584201.html

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Good morning

We are working on setting up an image screening assay in which we want to
measure apoptosis in one cell type (adherent)  after exposure to T cells
(non adherent). the adherent cells are grown on a monolayer and the T cells
are added on top and incubated for a few hrs (~3-5 hrs). We want to study
apoptosis in the adherent cells without interference from the T cells.
CellEvent (Thermo Fisher) works great in other conditions and is ideal for
a screening assay, but in this case it does not work because of the
presence of the Tcells, We would like to know is there is an alternative
indicator we can load in the adherent cells and wash prior to the T cells.
We need to test multiple cell lines so fluorescent protein based indicators
would not be ideal.

Thanks in advance for your help.

Leoncio Vergara MD
Co-Director

Center for Advanced Imaging (CAI) at the

Center for Translational Cancer Research (CTCR),

Institute for Biosciences and Technology,

Texas A&M Health Sciences Center,

Houston, Texas 77030