DIY phase contrast annulus

Posted by Smith, Benjamin E. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIY-phase-contrast-annulus-tp7584253.html

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Hey Microscopists,
    A lab here has a nice phase objective but no annulus, so I decided to see if I could make one for them.  I measured the phase plate diameter at the focal plane, and printed out an annulus that lined-up perfectly.  However, too much light gets through the ink to be of much use, so I was wondering if anyone had experience with an easy way to make a fully opaque annulus.

Cheers,
   Ben Smith

Benjamin E. Smith, Ph.D.
Samuel Roberts Noble Microscopy Laboratory
Research Scientist, Confocal Facility Manager
University of Oklahoma
Norman, OK 73019
E-mail: [hidden email]
Voice   405-325-4391
FAX  [phone]
http://www.microscopy.ou.edu/