Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIY-phase-contrast-annulus-tp7584253p7584254.html
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Have you considered printing multiple copies and stacking them to increase
contrast of the ring?
Craig
On Mon, Sep 28, 2015 at 5:53 PM, Smith, Benjamin E. <
[hidden email]>
wrote:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> Hey Microscopists,
> A lab here has a nice phase objective but no annulus, so I decided to
> see if I could make one for them. I measured the phase plate diameter at
> the focal plane, and printed out an annulus that lined-up perfectly.
> However, too much light gets through the ink to be of much use, so I was
> wondering if anyone had experience with an easy way to make a fully opaque
> annulus.
>
> Cheers,
> Ben Smith
>
> Benjamin E. Smith, Ph.D.
> Samuel Roberts Noble Microscopy Laboratory
> Research Scientist, Confocal Facility Manager
> University of Oklahoma
> Norman, OK 73019
> E-mail:
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> Voice 405-325-4391
> FAX 405-325-7619
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http://www.microscopy.ou.edu/