http://confocal-microscopy-list.275.s1.nabble.com/DIY-phase-contrast-annulus-tp7584253p7584257.html
I've made them with black varnish on an old phase-plate. Mount in
On 9/28/15 9:29 PM, JOEL B. SHEFFIELD wrote:
> *****
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> *****
>
> We have made Hoffman condensers using aluminum foil. I see no reason that
> you couldn't do something similar, using the mask that you have generated,
> to carefully cut out the ring in a sheet of aluminum that is already tacked
> down to a piece of glass.
>
> Joel
>
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail:
[hidden email]
> URL: *
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>
> On Mon, Sep 28, 2015 at 8:56 PM, Rusty Nicovich <
[hidden email]>
> wrote:
>
>> *****
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>> *****
>>
>> Ben,
>>
>> You could use chemical etching to reproduce your printed annulus on a piece
>> of copper or brass. Electronics DIYers make PC boards by laser printing a
>> pattern onto glossy paper, using an iron to transfer the toner to a piece
>> of copper-clad PC board, and the ferric chloride to etch away the copper.
>> Same principle will work on brass. Takes some trial and error and a few
>> nasty chemicals, but is a great way to get high-resolution parts out of
>> sheet metal.
>>
>> Another option is to have the annulus laser-cut. Shouldn't cost more than
>> $20 for something annulus-sized. Usually you need to supply the operator
>> with an Adobe Illustrator or other vector drawing format but you already
>> have the drawing. The cutter here is run by the architecture design school
>> and their cutter has a resolution of ~300 microns. Should be able to get
>> stainless steel or even powder-coated aluminum cut without too much
>> hassle. This would be my preference since it requires less OH&S work on my
>> part.
>>
>> Rusty
>>
>> On Tue, Sep 29, 2015 at 9:53 AM, Smith, Benjamin E. <
[hidden email]
>> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>>
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>> posting.
>>> *****
>>>
>>> Hey Microscopists,
>>> A lab here has a nice phase objective but no annulus, so I decided to
>>> see if I could make one for them. I measured the phase plate diameter at
>>> the focal plane, and printed out an annulus that lined-up perfectly.
>>> However, too much light gets through the ink to be of much use, so I was
>>> wondering if anyone had experience with an easy way to make a fully
>> opaque
>>> annulus.
>>>
>>> Cheers,
>>> Ben Smith
>>>
>>> Benjamin E. Smith, Ph.D.
>>> Samuel Roberts Noble Microscopy Laboratory
>>> Research Scientist, Confocal Facility Manager
>>> University of Oklahoma
>>> Norman, OK 73019
>>> E-mail:
[hidden email]
>>> Voice 405-325-4391
>>> FAX 405-325-7619
>>>
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.ou.edu_&d=BQIBaQ&c=82NFg6qkAwmLf-ElsTDuMzmUibVPgLssK8WcsyJgSiA&r=D67eBmNxz222Vdk0xqrsRlq4A21dYeahvvFbxFeCvQc&m=z2XJWKDxo9uW6sTc_-WVXTyJF8PmW5twCQnydbx1UoA&s=p_0y79O5jdi7KzRZbwmTjiLndLyC_vBP36Ei5qFliIA&e=Julian P.S. Smith III
Dept. of Biology