http://confocal-microscopy-list.275.s1.nabble.com/DIY-phase-contrast-annulus-tp7584253p7584260.html
microscope accessory ($80?)... just sayin ... don't want to ruin the fun
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> Sender: Confocal Microscopy List <
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> Poster: Leoncio Vergara <
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> Subject: Re: DIY phase contrast annulus
>
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>
> Seems a lot of high salaried personnel spending time on a relatively cheap
> microscope accessory ($80?)... just sayin ... don't want to ruin the fun
>
> On Tue, Sep 29, 2015 at 5:54 AM, Guy Cox <
[hidden email]> wrote:
>
> > This all seems really over the top. I made one simply by drawing it with
> > Indian ink on optical glass. I cleaned the pattern off an old one which
> > didn't match any objective I had, and used a Rotring pen to draw the
> inner
> > and outer circles with a compass, then inked in around them. Of course
> it
> > took care, but microscopists are surely used to delicate work. Cost -
> > zilch. This was 35 years ago - I still have it and anyone is welcome
> to
> > visit and try it.
> >
> > Guy
> >
> > Guy Cox, Honorary Associate Professor
> > School of Medical Sciences
> >
> > Australian Centre for Microscopy and Microanalysis,
> > Madsen, F09, University of Sydney, NSW 2006
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:
[hidden email]]
> > On Behalf Of JOEL B. SHEFFIELD
> > Sent: Tuesday, 29 September 2015 11:29 AM
> > To:
[hidden email]
> > Subject: Re: DIY phase contrast annulus
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
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> posting.
> > *****
> >
> > We have made Hoffman condensers using aluminum foil. I see no reason
> that
> > you couldn't do something similar, using the mask that you have
> generated,
> > to carefully cut out the ring in a sheet of aluminum that is already
> tacked
> > down to a piece of glass.
> >
> > Joel
> >
> >
> >
> > Joel B. Sheffield, Ph.D
> > Department of Biology
> > Temple University
> > Philadelphia, PA 19122
> > Voice: 215 204 8839
> > e-mail:
[hidden email]
> > URL: *
http://tinyurl.com/khbouft <
http://tinyurl.com/khbouft>*
> >
> > On Mon, Sep 28, 2015 at 8:56 PM, Rusty Nicovich <
[hidden email]
> >
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > Post images on
http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Ben,
> > >
> > > You could use chemical etching to reproduce your printed annulus on a
> > > piece of copper or brass. Electronics DIYers make PC boards by laser
> > > printing a pattern onto glossy paper, using an iron to transfer the
> > > toner to a piece of copper-clad PC board, and the ferric chloride to
> > etch away the copper.
> > > Same principle will work on brass. Takes some trial and error and a
> > > few nasty chemicals, but is a great way to get high-resolution parts
> > > out of sheet metal.
> > >
> > > Another option is to have the annulus laser-cut. Shouldn't cost more
> > > than
> > > $20 for something annulus-sized. Usually you need to supply the
> > > operator with an Adobe Illustrator or other vector drawing format but
> > > you already have the drawing. The cutter here is run by the
> > > architecture design school and their cutter has a resolution of ~300
> > > microns. Should be able to get stainless steel or even powder-coated
> > > aluminum cut without too much hassle. This would be my preference
> > > since it requires less OH&S work on my part.
> > >
> > > Rusty
> > >
> > > On Tue, Sep 29, 2015 at 9:53 AM, Smith, Benjamin E.
> > > <
[hidden email]
> > > >
> > > wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > > Post images on
http://www.imgur.com and include the link in your
> > > posting.
> > > > *****
> > > >
> > > > Hey Microscopists,
> > > > A lab here has a nice phase objective but no annulus, so I
> > > > decided to see if I could make one for them. I measured the phase
> > > > plate diameter at the focal plane, and printed out an annulus that
> > lined-up perfectly.
> > > > However, too much light gets through the ink to be of much use, so I
> > > > was wondering if anyone had experience with an easy way to make a
> > > > fully
> > > opaque
> > > > annulus.
> > > >
> > > > Cheers,
> > > > Ben Smith
> > > >
> > > > Benjamin E. Smith, Ph.D.
> > > > Samuel Roberts Noble Microscopy Laboratory Research Scientist,
> > > > Confocal Facility Manager University of Oklahoma Norman, OK 73019
> > > > E-mail:
[hidden email]
> > > > Voice 405-325-4391
> > > > FAX 405-325-7619
> > > >
http://www.microscopy.ou.edu/> > >
> >
>