http://confocal-microscopy-list.275.s1.nabble.com/Ex-Vivo-Fluorescent-Bone-Stain-tp7584365p7584366.html
even have to stain. Just hit the sample with twice the wavelength of
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> I'm very new to microscopy, so I apologize in advance if this is the wrong place to be posing
> this question:
>
> I've been struggling to find a fluorescent bone mineral stain that functions ex vivo. In the
> literature it seems that most stains (calcein, alizarin complexone, xylenol orange etc.) must
> be injected in vivo to work properly – this is also what people in the lab have been telling
> me.
>
> Because I'm not familiar with how these stains work, I'm wondering if there is a fluorescent
> stain that can be applied to mineralized tissue ex vivo (not necessarily cells, just the
> mineralized matrix)?
>
> I am trying to differentiate mineralized tissue from soft tissue of frozen, then thawed
> samples using two-photon excitation microscopy.
>
> Any help would be greatly appreciated. Thank you!
>
> Johnathan Sevick, BASc| MSc Candidate
> Biomedical Engineering Graduate Program | University of Calgary
> Email:
[hidden email]
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Biomedical Center (BMC)
> Head of the Core Facility Bioimaging
>
> Großhaderner Straße 9
> D-82152 Planegg-Martinsried
> Germany